Oligotex™-dT30 Super






Oligotex™-dT30 Superはラテックス粒子の表面にOligo(dT)30の5'末端領域を結合させたポリマー微粒子の分散液で、熱、アルカリに安定です。
Oligotex™ 粒子は表面積が大きく、懸濁性が高いことから、固定化されたOligo(dT)30と試料中のPoly(A)+―mRNAは迅速かつ効率よくハイブリッド反応します。
得られたmRNA-Oligotex™-dT30 Super複合体は、3分程度の遠心分離で容易に分離できます。
また、mRNA-Oligotex™-dT30 Super複合体のままで、RT-PCRに利用できます。




粒子固形分:10%(w/v) 分散液:TE/0.1%SDS/0.1% NaN3




Oligotex™-dT30 Super を用いたtotal RNAからのmRNAの精製例


HeLa細胞から採取した精製前のtotal RNA




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